Two EcoRI RFLPs at the GLUT2 locus

Two RFLPs at the TNP1 locus.

Source/Description: The Human Transition Protein 1 gene (TNP1) probe (1) used was a 700 bp genomic DNA sequence cloned into the Smal site of pUC8 and was supplied by W.Engel. Polymorphisms: TNP1 identifies a two allele polymorphism with TaqI: Al = 9.6 kb; A2 = 8.4 kb. Frequency: Analysis of 20 unrelated individuals yielded frequencies: Al = 0.23; A2 = 0.77. Rsal in a screen of 10-20 unrelated i...

Three RFLPs for the insulin receptor gene INSR: EcoRI, Pst I, Hind III.

SOURCE AND DESCRIPTION OF CLONE: A 973 bp cDNA insert from an adult human kidney library cloned in the EcoRI site of PBR 327 (pINSR 18.2) encoding aminoacids-26 to 295 of the insulin receptor. POLYMORPHISM EcoRI identifies a two allele polymorphism with bands at 22 fcb and 20 Kb Hind III detects two allelic fragments of 6.5 Kb and 1.4 kb Pst I reveals two allelic fragments of 2.

RFLPs at the D19S19 locus of human chromosome 19 linked to myotonic dystrophy (DM)

SOURCE/DESCRIPTION: Probe LDR152-a 1.68 Kb single copy Hind III fragment isolated from a chromosome 19 bivariate flow sorted enriched Charon 21A library and subcloned into SP64. CHROMOSOMAL LOCALISATION: 1 9cent-1 9q 1 • 3 by somatic cell hybrid and linkage data. POLYMORPHISMS: Recognises two, two allele polymorphisms when U3ed to probe human genomic DNA digested with P3t I and Msp I.

Rapid communication: an EcoRI polymorphism at the porcine nebulin (Neb) locus.

An EcoRI polymorphism at the insulin receptor locus on a fragment comprising exons 4 to 8.

A 593 bp EcoRI-Bgll fragment encompassing bp 1012 to 1604 of the human insulin receptor cDNA (1). Polymorphism: EcoRI detects a two allele polymorphism with bands at 10.3 and 13.5 kb (Fig. 1. Lanes 1—4 shows three homozygotes and one heterozygote). Segregation: Segregation of alleles in tumors with 19p+ marker chromosomes (2) (Fig. 1). Lane 5 shows tumor DNA with duplicated 19p+ marker chromoso...